التحليل الطيفي الكمي لهيدروكلوريد الميتوكلوبراميد في الأقراص الصيدلانية عبر تفاعل البرومة
DOI:
https://doi.org/10.67151/rujbas.v1i1.29الكلمات المفتاحية:
هيدروكلوريد الميتوكلوبراميد، خليط البرومات والبروميد، أزرق الميثيلين، التحليل الطيفي، الأقراص الصيدلانيةالملخص
هدفت هذه الدراسة إلى اقتراح طريقة طيفية مرئية بسيطة وحساسة والتحقق من صحتها لتقدير كمية هيدروكلوريد الميتوكلوبراميد (MCPH) في شكله النقي وفي الأقراص الصيدلانية. تستخدم هذه الطريقة البروم المُنتَج في الموقع (in situ) بتأثير الحامض على خليط من البرومات والبروميد، والذي يعمل كعامل برومة صديق للبيئة لبرومة هيدروكلوريد الميتوكلوبراميد. تتيح هذه الطريقة تحليلًا منخفض التكلفة مع إلغاء الحاجة إلى التعامل مع البروم السائل الخطير والسام. اعتمدت الطريقة على إضافة زيادة مُقاسة بدقة من كاشف البرومات والبروميد إلى محلول الميتوكلوبراميد في وسط حامضي. وبعد اكتمال التفاعل، تم تقدير البروم المتبقي من خلال تفاعله مع كمية ثابتة من صبغة أزرق الميثيلين، ثم قياس الامتصاص عند طول موجي قدره 665 نانومتر. وفي الظروف المثلى، يمكن تقدير الميتوكلوبراميد ضمن نطاق تركيز يتراوح بين 1.25 و25.0 ميكروغرام/مل، بمعامل امتصاص مولاري قدره 10⁴×1.032 لتر/مول.سم وحساسية ساندل قدرها 0.0326 ميكروغرام/سم². وتم حساب قيم t وF العملية ومقارنتها بالقيم الجدولية الحرجة باستخدام اختبار الطالب t ونسبة F عند مستوى ثقة 95٪، مما أكد عدم وجود فروق جوهرية بين الطريقة المقترحة والطريقة المعتمدة من حيث الدقة والمصداقية. طُبقت الطريقة المقترحة بنجاح لتحليل الميتوكلوبراميد في المادة الخام وفي الأقراص، وتأكدت موثوقيتها باستخدام طريقة الإضافة القياسية.
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